|SELENITE BROTH BASE 500g
Selenite Broth Base 500g
Thermo ScientificTM OxoidTM
Enrich and isolate Salmonella from feces and food products with Thermo Scientific™ Oxoid™ Selenite Broth Base (Lactose).The presence of Selenium in the medium provides selectivity against non-target bacteria.
Selenite Broth Base (Lactose), Oxoid Composition
|pH 7.1 ± 0.2 @ 25°C
Selenite Broth Base (Lactose), Oxoid Preparation:
Dissolve 4g of sodium biselenite LP0121 in 1 litre of distilled water and then add 19g of Selenite Broth Base. Warm to dissolve, mix well and fill out into containers. Sterilise in a boiling water bath, or in free flowing steam, for 10 minutes. DO NOT AUTOCLAVE.
To minimise any possible risk of teratogenicity to laboratory workers, the sodium biselenite must be added as a solution to this medium.
Robertson1 reported miscarriages and possible teratogenic effects on pregnant laboratory assistants which may have been caused by ingested sodium biselenite. Oxoid therefore removed this substance from the powdered medium.
Although no further reports have been received sodium biselenite is now considered to be very toxic and should be handled with great care.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C away from light.
Discard the prepared medium if large amounts of reduced selenite can be seen as a red precipitate in the bottom of the bottles.
Do not incubate longer than 24 hours because the inhibitory effect of selenite is reduced after 6-12 hours incubation.
Take sub-cultures of broth from the upper third of the broth column, which should be at least 5 cm in depth.