Product Code | Description | Product Size | Quantity |
CM0627B | DEHYDRATED ROGOSA AGAR 500g | – | 500g |
Dehydrated Rogosa Agar 500g
Brand:
Thermo ScientificTM OxoidTM
Oxoid Rogosa Agar is used for the selective isolation and enumeration of lactobacilli.
Rogosa Agar, Oxoid Composition
Typical Formula* | gm/litre |
Tryptone | 10.0 |
Yeast extract | 5.0 |
Glucose | 20.0 |
Sorbitan mono-oleate `Tween 80’ | 1.0ml |
Potassium dihydrogen phosphate | 6.0 |
Ammonium citrate | 2.0 |
Sodium acetate, anhydrous | 17.0 |
Magnesium sulphate | 0.575 |
Manganese sulphate | 0.12 |
Ferrous sulphate | 0.034 |
Agar | 20.0 |
pH 5.4 ± 0.2 @ 25°C |
Rogosa Agar, Oxoid Preparation:
Suspend 82g in 1 litre of distilled water and bring to the boil to dissolve completely. Add 1.32ml glacial acetic acid and mix thoroughly. Heat to 90-100°C for 2-3 minutes with frequent agitation. Distribute into sterile tubes, Petri dishes or bottles.
DO NOT AUTOCLAVE.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
Lactobacillus carnis does not grow on this medium.
All colonies must be tested by Gram stain and catalase test before carrying out further identification tests.
Dimensions | 10 cm |
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Brand | Oxoid |
Estimated Delivery Time | 1-2 weeks |