|Product Code||Description||Product Size||Quantity|
|CM0979B||E.C. Broth with Mug 500g||–||500g|
E.C. Broth with Mug 500g
Thermo ScientificTM OxoidTM
Oxoid EC Broth with MUG is used for the isolation and presumptive identification of E. coli.
EC Broth with MUG, Oxoid Composition
|Bile salts No. 3|
|pH 6.9 ± 0.2 @ 25°C|
EC Broth with MUG, Oxoid Preparation:
Suspend 37g of EC Broth with MUG in 1 litre of distilled water. Mix well to dissolve and distribute into final containers containing inverted Durham tubes. Sterilise by autoclaving at 121° C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Ensure that the glass tubes used for the preparation of the medium do not auto fluoresce and take the relevant safety precautions when using a UV lamp.
Some pathogenic strains, typically Escherichia coli O157:H7 are usually glucuronidase negative and therefore will not give a positive result in this test.
Other enteric organisms e.g. some Shigella species are also glucuronidase positive.