| Product Code | Description | Product Size | Quantity |
| CM0979B | E.C. Broth with Mug 500g | – | 500g |
E.C. Broth with Mug 500g
Brand:
Thermo ScientificTM OxoidTM
Oxoid EC Broth with MUG is used for the isolation and presumptive identification of E. coli.
EC Broth with MUG, Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptone | 20.0 |
| Lactose | 5.0 |
| Bile salts No. 3 | 1.5 |
| Di-potassium phosphate | 4.0 |
| Mono-potassium phosphate | 1.5 |
| Sodium chloride | 5.0 |
| 4-methylumbelliferyl-ß-D-glucuronide (MUG) | 0.05 |
| pH 6.9 ± 0.2 @ 25°C |
EC Broth with MUG, Oxoid Preparation:
Suspend 37g of EC Broth with MUG in 1 litre of distilled water. Mix well to dissolve and distribute into final containers containing inverted Durham tubes. Sterilise by autoclaving at 121° C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
Ensure that the glass tubes used for the preparation of the medium do not auto fluoresce and take the relevant safety precautions when using a UV lamp.
Some pathogenic strains, typically Escherichia coli O157:H7 are usually glucuronidase negative and therefore will not give a positive result in this test.
Other enteric organisms e.g. some Shigella species are also glucuronidase positive.
100% Authentic Guarantee Description
Additional information
| Dimensions | 10 cm |
|---|---|
| Brand | Oxoid |
| Estimated Delivery Time | 1-2 weeks |
