Brand:
Thermo ScientificTM OxoidTM
Oxoid EC Broth with MUG is used for the isolation and presumptive identification of E. coli.
EC Broth with MUG, Oxoid Composition
| Typical Formula* |
gm/litre
|
| Tryptone |
20.0
|
| Lactose |
5.0
|
| Bile salts No. 3 |
1.5
|
| Di-potassium phosphate |
4.0
|
| Mono-potassium phosphate |
1.5
|
| Sodium chloride |
5.0
|
| 4-methylumbelliferyl-Ã-D-glucuronide (MUG) |
0.05
|
| pH 6.9 ± 0.2 @ 25°C |
|
EC Broth with MUG, Oxoid Preparation:
Suspend 37g of EC Broth with MUG in 1 litre of distilled water. Mix well to dissolve and distribute into final containers containing inverted Durham tubes. Sterilise by autoclaving at 121° C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
Ensure that the glass tubes used for the preparation of the medium do not auto fluoresce and take the relevant safety precautions when using a UV lamp.
Some pathogenic strains, typically Escherichia coli O157:H7 are usually glucuronidase negative and therefore will not give a positive result in this test.
Other enteric organisms e.g. some Shigella species are also glucuronidase positive.