Histopaque®-1077 (Sigma-Aldrich)

General description

Histopaque-1077 is a sterile, endotoxin tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077g/mL. This ready-to-use medium facilitates rapid recovery of viable lymphocytes and other mononuclear cells from small volumes of whole blood.

Application

Haematology: suitable
Histology: suitable

Histopaque-1077 has been used for-
• the isolation of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)
• purification of mononuclear cells through centrifugation
• isolation of human lymphocytes
• isolation of breast cancer circulating tumour cells (CTCs) from whole blood