Brand:
Thermo ScientificTM OxoidTM
Isolate and enumerate lactic acid bacteria from food with Thermo Scientific⢠Oxoid⢠MRS Broth (DeMan, Rogosa and Sharpe) (Dehydrated). This non-selective medium is designed to support profuse growth of lactic acid bacteria.
MRS Broth (DeMan, Rogosa and Sharpe), Oxoid Composition
| Typical Formula* | gm/litre |
| Peptone | 10.0 |
| `Lab-Lemcoâ powder | 8.0 |
| Yeast extract | 4.0 |
| Glucose | 20.0 |
| Sorbitan mono-oleate | 1 ml |
| Dipotassium hydrogen phosphate | 2.0 |
| Sodium acetate 3H2O | 5.0 |
| Triammonium citrate | 2.0 |
| Magnesium sulphate 7H2O | 0.2 |
| Manganese sulphate 4H2O | 0.05 |
| pH 6.2 ± 0.2 @ 25°C | |
MRS Broth (DeMan, Rogosa and Sharpe), Oxoid Preparation:
Add 52g to 1 litre of distilled water at approximately 60°C. Mix until completely dissolved. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.