Thermo ScientificTM OxoidTM
Cultivate lactic streptococci and their bacteriophages with Thermo Scientific⢠Oxoid⢠M17 Broth (Dehydrated). The use of M17 Broth in conjunction with M17 Agar is recommended by Terzaghi and Sandine for bacteriophage assays.
M17 Broth, Oxoid Composition
| Typical Formula* |
gm/litre |
| Tryptone |
5.0 |
| Soya peptone |
5.0 |
| `Lab-Lemcoâ powder |
5.0 |
| Yeast extract |
2.5 |
| Ascorbic acid |
0.5 |
| Magnesium sulphate |
0.25 |
| Di-sodium-glycerophosphate |
19.0 |
| pH 6.9 ± 0.2 @ 25°C |
|
M17 Broth, Oxoid Preparation:
Suspend 37.25g in 950ml of distilled water and bring gently to the boil. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 50ml of sterile lactose solution (10% w/v).
Lactose solution 10% (w/v):
Dissolve 10g of Lactose Code LP0070 in 100ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes or by membrane filtration through a 0.2mm membrane.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
| Specification | Chair | Armchair | Sofas |
| Height | 37" | 42" | 42" |
| Width | 26.5" | 32.5" | 142" |
| Depth | 19.5" | 22.5" | 24.5" |
| Assembly Required | No | No | Yes |
| Packaging Type | Box | Box | Box |
| Package Weight | 55 lbs. | 64 lbs. | 180 lbs. |
| Packaging Dimensions | 27" x 26" x 39" | 45" x 35" x 24" | 46" x 142" x 25" |
| Product Code | Description | Product Size | Quantity |
| CM0817B | M17 Broth 500g | – | 500g |
| Dimensions | 10 cm |
|---|---|
| Brand | Oxoid |
| Estimated Delivery Time | 1-2 weeks |
