Mineral Modified Medium + Sodium Glutamate

Identify members of the coliform group of bacteria in food and water samples with Thermo Scientific™ Oxoid™ Minerals Modified Glutamate Medium (Dehydrated). The mineral content of the medium allows for the superior cultivation and enumeration of coliforms.

Minerals Modified Glutamate Medium, Oxoid Composition

Minerals Modified Glutamate Medium, Oxoid Preparation:
Double Strength:
Dissolve 5g of ammonium chloride in 1 litre of distilled water. To this add 22.7g of Minerals Modified Medium Base, and 12.7g of Sodium glutamate LP0124. Mix to dissolve completely. Sterilise by autoclaving for 10 minutes at 116°C; alternatively heat to 100°C for 30 minutes on three successive days.

Single strength:
Dissolve 2.5g of ammonium chloride in 1 litre of distilled water. To this add 11.4g of Minerals Modified Medium Base, and 6.4g of Sodium glutamate LP0124. Mix to dissolve completely. Sterilise by autoclaving for 10 minutes at 116°C; alternatively heat to 100°C for 30 minutes on three successive days.

Note
To improve the stability of the dehydrated medium on storage the sodium glutamate LP0124 is supplied separately and must be added to the basal medium CM0607.

The pH of the final medium is critical for optimum performance and the sterilised broth should be checked to confirm that it is at pH 6.7 before use.

Differences in heating procedures cause differences in final pH value. If necessary the heating procedure should be adjusted so that the final pH, after sterilisation is 6.7

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Presumptive positive tubes must be sub-cultured to Lauryl Tryptose Mannitol Broth CM0831 and incubated at 44°C to detect indole formation at this temperature before the identification of Escherichia coli can be made.