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HomeMicrobiologyCulture Media Products MKTTn BROTH BASE (ISO) 500g
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MKTTn BROTH BASE (ISO) 500g

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Thermo ScientificTM OxoidTM

Selectively isolate Salmonella species from food samples with Thermo Scientific™ Muller-Kauffmann Tetrathionate-Novobiocin Broth (MkTTn) for food testing in compliance with ISO 6579:2002.

Muller-Kauffmann Tetrathionate-Novobiocin Broth (MkTTn), Oxoid Composition

Typical Formula*
gm/litre
Meat extract

4.3

Enzymatic digest of casein

8.6

Sodium chloride
2.6
Calcium carbonate
38.7
Sodium thiosulphate (anhydrous)
30.5†
Ox bile
4.78
Brilliant green
0.0096
pH 8.0 ± 0.2 @ 25°C

 

Muller-Kauffmann Tetrathionate-Novobiocin Broth (MkTTn), Oxoid Preparation:
Suspend 89.5g of MKTTn in 1 litre of distilled water, mix well and bring to the boil. Cool to below 45°C. Immediately before use add 20ml of iodine-iodide solution prepared by dissolving 25g of potassium iodide in 10ml of water, adding 20g of iodine and then diluting to 100ml with sterile water. Also add the contents of four vials of Oxoid Novobiocin Selective Supplement SR0181 reconstituted as directed. Mix well and aseptically dispense into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared media at room temperature. The prepared medium in its final form containing iodine/iodide solution + Novobiocin Selective Supplement SR0181 should be used immediately.

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SKU: CM1048B Categories: Culture Media Products, Microbiology
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Description
Product CodeDescriptionProduct SizeFill VolumeFormat
CM1048BMkttn Broth Base (ISO) 500g–500gPowder
Additional information
Dimensions10 cm
Brand

Oxoid

Estimated Delivery Time

1-2 weeks

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MKTTn BROTH BASE (ISO) 500g

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