Brand:
Thermo ScientificTM OxoidTM
Oxoid MRS Agar (de Man, Rogosa, Sharpe) (Dehydrated) is used for the growth of Lactobacilli.
Composition of MRS Agar, Oxoid
| Typical Formula * | gm/litre |
| Peptone | 10.0 |
| `Lab-Lemcoâ powder | 8.0 |
| Yeast Extract | 4.0 |
| Glucose | 20.0 |
| Sorbitan mono-oleate | 1ml |
| Dipotassium hydrogen phosphate | 2.0 |
| Sodium acetate 3H2O | 5.0 |
| Triammonium citrate | 2.0 |
| Magnesium sulphate 7H2O | 0.2 |
| Manganese sulphate 4H2O | 0.05 |
| Agar | 10.0 |
| pH 6.2 ± 0.2 @ 25°C | |
Preparation of MRS Agar, Oxoid
Suspend 62g of your MRS Agar in 1 litre of distilled water. You can boil to dissolve the powder completely. After a dissolve, you can sterilise the medium by autoclaving at 121°C for 15 minutes.
Storage Conditions and Shelf Life of MRS Agar, Oxoid
You can store your dehydrated MRS Agar at 10-30°C and use before the expiry date on the label. You can store the prepared plates at 2-8°C.