Brand:
Thermo ScientificTM OxoidTM
Oxoid MRS Agar (de Man, Rogosa, Sharpe) (Dehydrated) is used for the growth of Lactobacilli.
Composition of MRS Agar, Oxoid
| Typical Formula * |
gm/litre
|
| Peptone |
10.0
|
| `Lab-Lemcoâ powder |
8.0
|
| Yeast Extract |
4.0
|
| Glucose |
20.0
|
| Sorbitan mono-oleate |
1ml
|
| Dipotassium hydrogen phosphate |
2.0
|
| Sodium acetate 3H2O |
5.0
|
| Triammonium citrate |
2.0
|
| Magnesium sulphate 7H2O |
0.2
|
| Manganese sulphate 4H2O |
0.05
|
| Agar |
10.0
|
| pH 6.2 ± 0.2 @ 25°C |
|
Preparation of MRS Agar, Oxoid
Suspend 62g of your MRS Agar in 1 litre of distilled water. You can boil to dissolve the powder completely. After a dissolve, you can sterilise the medium by autoclaving at 121°C for 15 minutes.
Storage Conditions and Shelf Life of MRS Agar, Oxoid
You can store your dehydrated MRS Agar at 10-30°C and use before the expiry date on the label. You can store the prepared plates at 2-8°C.