Thermostable RNase H, NEB

Thermostable RNase H from NEB specifically recognizes and cleaves the phosphodiester bonds of an RNA strand in an RNA-DNA hybrid while leaving the DNA strand intact. This thermostable nuclease exhibits the same enzymatic properties as E. coli RNase H, but is active at much higher temperatures.

Features of Thermostable RNase H, NEB

• Recombinant enzyme supplied with 10X Reaction Buffer
• Does not digest single or double-stranded DNA
• Active and stable above 37°C over a broad temperature range, with activity increasing with temperature
• Requires magnesium chloride for activity
• Inactivated with proteinase K treatment or addition of excess EDTA