Showing all 13 results

Show sidebar

APE 1

RM399.00RM1,499.00
Brand:

New England Biolabs

  • Apurinic/apyrimidinic Endonuclease 1
  • DNA AP endonuclease that catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3′-hydroxyl and 5′ deoxyribose phosphate (dRP) termini
  • APE 1 has also been reported to have DNA 3′-diesterase, 3′ to 5′ exonuclease, and RNase H activities
  • Also known as HAP1 or Ref-1

Blunt/TA Ligase Master Mix

RM523.00RM1,993.00
Brand:

New England Biolabs

Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer. This master mix is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates.

  • Simplifies reaction set-up, ensures an optimized ratio of enzyme and buffer components
  • No thawing is necessary as it remains liquid during storage at -20°C
  • Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution
  • Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart

Cas9 Nuclease, S. pyogenes

RM283.00RM2,733.00
Brand:

New England Biolabs

DNase I (RNase-free)

RM383.00RM1,443.00
Brand:

New England Biolabs

  • Endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5′-phosphorylated and 3′-hydroxylated ends
  • Degrades DNA template in transcription reactions
  • Removes contaminating genomic DNA from RNA samples
  • Isolated from a recombinant source

ElectroLigase – 50 rxns

RM639.00
Brand:

New England Biolabs

  • Directly compatible with electrocompetent cells
  • No thawing needed as it maintains liquid form at -20°C
  • Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart

EnGen Cas9 NLS, S. pyogenes

RM783.00RM3,033.00
Brand:

New England Biolabs

This product was formerly named EnGen Cas9 NLS, S. pyogenes.

  • Ideal for direct introduction of Cas9/sgRNA complexes
  • Dual NLS for improved transport to the nucleus
  • Compatible with EnGen® sgRNA Synthesis Kit, S. pyogenes (NEB #E3322S) and the EnGen Mutation Detection Kit (NEB #E3321S)

EnGen Spy Cas9 Nickase

RM333.00RM783.00
Brand:

New England Biolabs

  • Variant of Cas9 nuclease differing by a point mutation (D10A) in the RuvC nuclease domain
  • Capable of generating nicks, but not cleaving DNA
  • DNA double strand breaks can be generated, with reduced off-target cleavage, by targeting two sites with EnGen Cas9 Nickase in close proximity

Instant Sticky-end Ligase Master Mix

RM513.00RM1,953.00
Brand:

New England Biolabs

Specifically formulated to rapidly ligate cohesive-end (2–4 bp) substrates and improve transformation, Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA Ligase and a proprietary ligation enhancer in an optimized reaction buffer.

  • No incubation time necessary to achieve ligation
  • Efficiencies sufficient for successful sub-cloning of sticky-end substrates
  • No thawing of the master mix is required as liquid state is maintained when stored at -20°C
  • Simplified reaction setup with optimized ratio of enzyme and buffer components
  • Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution
  • Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart

Quick CIP, NEB

RM483.00RM2,183.00
Brand:

New England Biolabs

Quick CIP from NEB is a heat-labile version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source.

Features of Quick CIP, NEB:

  • Rapid and irreversible heat inactivation eliminates the unwanted activity
  • Improved storage stability versus native enzyme
  • Faster reaction setup (no supplemental additives like zinc required) and shorter incubation time
  • Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)
  • Less enzyme required (high specific activity), resulting in a lower cost per reaction
  • No need for multiple phosphatases (Quick CIP removes 5′- and 3′- phosphates from DNA, RNA and dNTPs )
  • Active on unincorporated dNTPs in PCR products – improve DNA sequencing and SNP analysis
  • Recombinant for purity, consistency and value

Quick Ligation Kit

RM519.00RM1,973.00
Brand:

New England Biolabs

The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25°C )

  • Fast – 5 minutes for cohesive or blunt ends
  • Convenient – ligation performed at room temperature
  • Flexible – suitable for all common ligation reactions
  • Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart

Shrimp Alkaline Phosphatase (rSAP)

RM343.00RM1,273.00
Brand:

New England Biolabs

Shrimp Alkaline Phosphatase (rSAP) is a heat labile alkaline phosphatase purified from a recombinant source.

  • Rapid and irreversible heat inactivation eliminates unwanted activity
  • Improved storage stability versus native enzyme
  • Faster reaction setup (no supplemental additives like zinc required)
  • Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)
  • Less enzyme required (high specific activity), resulting in a lower cost per reaction
  • No need for multiple phosphatases (rSAP removes 5´- and 3´- phosphates from DNA, RNA and dNTPs )
  • Active on unincorporated dNTPs in PCR products – improves DNA sequencing and SNP analysis
  • Recombinant for purity, consistency and value

T4 DNA Ligase, NEB

RM353.00RM1,313.00
Brand:

New England Biolabs

Characteristics of T4 DNA Ligase, NEB:

  • Catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA.
  • This enzyme will join blunt end and cohesive end termini as well as repair single-stranded nicks in duplex DNA and some DNA/RNA hybrids (1).
  • T4 DNA ligase will seal nicks for these DNA substrates.
  • The industry standard for performance and quality
  • Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart

Thermostable RNase H, NEB

RM739.00
Brand:

New England Biolabs

Thermostable RNase H from NEB specifically recognizes and cleaves the phosphodiester bonds of an RNA strand in an RNA-DNA hybrid while leaving the DNA strand intact. This thermostable nuclease exhibits the same enzymatic properties as E. coli RNase H, but is active at much higher temperatures.

Features of Thermostable RNase H, NEB

  • Recombinant enzyme supplied with 10X Reaction Buffer
  • Does not digest single or double-stranded DNA
  • Active and stable above 37°C over a broad temperature range, with activity increasing with temperature
  • Requires magnesium chloride for activity
  • Inactivated with proteinase K treatment or addition of excess EDTA