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HomeCell Biology Trypsin from Porcine Pancreas (Sigma)
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Trypsin from Porcine Pancreas (Sigma)

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Brand:

Sigma

Description

Product line: BioReagent

Biological source: Porcine pancreas

Specific activity: 1,000-2,000 BAEE units/mg solid

Trypsin is applicable for tissue disaggregation, due to its effective action and tolerance towards different cell types and serum-induced neutralization.[1]

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestionsns†. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.
Trypsin from porcine pancreas has been used to digest chicken bones.[2] It has also been used in the isolation of luteal endothelial cells.[3][4][5]
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Description
Product CodeProduct NamePack SizeFormStorage Temperature
T4799-5GTrypsin5GLyophilized powder-20ºC
T4799-10GTrypsin10GLyophilized powder-20ºC
T4799-25GTrypsin25GLyophilized powder-20ºC
Additional information
DimensionsN/A
Brand

Sigma

Delivery Time

3 – 5 weeks

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Trypsin from Porcine Pancreas (Sigma)

Trypsin from Porcine Pancreas (Sigma)

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