Urea Broth Base 500g

Oxoid Urea Broth Base (Christensen Broth Base) is used for the detection of urease producing microorganisms.

Urea Broth Base, Oxoid Composition

Urea Broth Preparation:
Add 0.9 g to 95 ml of distilled water. Sterilise by autoclaving at 115°C for 20 minutes. Cool to 55°C and aseptically introduce 5 ml of sterile 40% Urea Solution SR0020. Mix well and distribute 10 ml amounts into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
It is preferable that the medium be used on the day of preparation. If not, examine the tubes carefully to ensure sterility.
After overnight incubation other members of the Enterobacteriaceae may show alkaline reactions.