Brand:
Thermo ScientificTM OxoidTM
- Oxoid WL Nutrient Agar is used for the determination of the microbiological flora in brewing and fermentation processes.
- Also available Cycloheximide 0.1% Solution, Part No. SR0222C.
WL Nutrient Agar, Oxoid Composition
| Typical Formula* |
gm/litre
|
| Yeast extract |
4.0
|
| Tryptone |
5.0
|
| Glucose |
50.0
|
| Potassium dihydrogen phosphate |
0.55
|
| Potassium chloride |
0.425
|
| Calcium chloride |
0.125
|
| Magnesium sulphate |
0.125
|
| Ferric chloride |
0.0025
|
| Manganese sulphate |
0.0025
|
| Bromocresol green |
0.022
|
| Agar |
15.0
|
| pH 5.5 ± 0.2 |
|
WL Nutrient Agar, Oxoid Preparation:
Suspend 75g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. If required the pH may be adjusted to 6.5 by the addition of 1% sodium bicarbonate solution.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
When handling cycloheximide observe the precautions to be taken under HAZARDS.