Brand:
Thermo ScientificTM OxoidTM
- Oxoid WL Nutrient Agar is used for the determination of the microbiological flora in brewing and fermentation processes.
- Also available Cycloheximide 0.1% Solution, Part No. SR0222C.
WL Nutrient Agar, Oxoid Composition
| Typical Formula* | gm/litre |
| Yeast extract | 4.0 |
| Tryptone | 5.0 |
| Glucose | 50.0 |
| Potassium dihydrogen phosphate | 0.55 |
| Potassium chloride | 0.425 |
| Calcium chloride | 0.125 |
| Magnesium sulphate | 0.125 |
| Ferric chloride | 0.0025 |
| Manganese sulphate | 0.0025 |
| Bromocresol green | 0.022 |
| Agar | 15.0 |
| pH 5.5 ± 0.2 | |
WL Nutrient Agar, Oxoid Preparation:
Suspend 75g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. If required the pH may be adjusted to 6.5 by the addition of 1% sodium bicarbonate solution.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
When handling cycloheximide observe the precautions to be taken under HAZARDS.