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E.E. Broth 500g

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Thermo ScientificTM OxoidTM

Enrich Enterobacteriaceae in bacteriological examination of foods and animal feed stuffs with Thermo Scientific™ Oxoid™ EE Broth (Buffered Glucose Brilliant Green Bile Broth) (Dehydrated). This medium is more inhibitory to non-Enterobacteriaceae than other non-selective media e.g. Mannitol broth or Lactose broth by virtue of the presence of brilliant green and bile salts in the preparation.

EE Broth, Oxoid Composition

Typical Formula* 

gm/litre

Peptone

10.0

Glucose

5.0

Disodium hydrogen phosphate anhyd.

6.45

Potassium dihydrogen phosphate

2.0

Ox Bile purified

20.0

Brilliant green

0.0135

pH 7.2 ± 0.2 @ 25°C

 

EE Broth, Oxoid Preparation:
Add 43.5g to 1 litre of distilled water. Distribute 100ml quantities in 250ml flasks and heat at 100°C for 30 minutes only. Cool rapidly in cold running water. This medium is heat sensitive. DO NOT AUTOCLAVE.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Avoid overheating the medium, especially the double-strength broth.

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SKU: CM0317B Categories: Culture Media Products, Microbiology
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  • Additional information
Description
Product CodeDescriptionProduct SizeQuantity
CM0317BE.E. BROTH 500g–500g
Additional information
Dimensions10 cm
Brand

Oxoid

Estimated Delivery Time

1-2 weeks

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E.E. Broth 500g

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