|E.E. BROTH 500g
E.E. Broth 500g
Thermo ScientificTM OxoidTM
Enrich Enterobacteriaceae in bacteriological examination of foods and animal feed stuffs with Thermo Scientific™ Oxoid™ EE Broth (Buffered Glucose Brilliant Green Bile Broth) (Dehydrated). This medium is more inhibitory to non-Enterobacteriaceae than other non-selective media e.g. Mannitol broth or Lactose broth by virtue of the presence of brilliant green and bile salts in the preparation.
EE Broth, Oxoid Composition
|Disodium hydrogen phosphate anhyd.
|Potassium dihydrogen phosphate
|Ox Bile purified
|pH 7.2 ± 0.2 @ 25°C
EE Broth, Oxoid Preparation:
Add 43.5g to 1 litre of distilled water. Distribute 100ml quantities in 250ml flasks and heat at 100°C for 30 minutes only. Cool rapidly in cold running water. This medium is heat sensitive. DO NOT AUTOCLAVE.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Avoid overheating the medium, especially the double-strength broth.