Bolton Broth Selective Supplement

Oxoid Bolton Broth Selective Supplement is used for the selective pre-enrichment of Campylobacter spp. in food samples.

Add to to Bolton Broth, Part No. CM0983B. Each vial supplements 500mL of medium.

Bolton Broth Selective Supplement, Oxoid Composition

Bolton Broth, Oxoid Preparation:
Add 13.8g of Bolton Broth to 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml Laked Horse Blood SR0048 and 1 vial of Bolton Broth Selective Supplement SR0183, reconstituted as directed. Mix well and distribute into sterile screw top containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for up to 2 weeks.