Product Code | Description | Product Size | Quantity |
CM0317B | E.E. BROTH 500g | – | 500g |
E.E. Broth 500g
Brand:
Thermo ScientificTM OxoidTM
Enrich Enterobacteriaceae in bacteriological examination of foods and animal feed stuffs with Thermo Scientific™ Oxoid™ EE Broth (Buffered Glucose Brilliant Green Bile Broth) (Dehydrated). This medium is more inhibitory to non-Enterobacteriaceae than other non-selective media e.g. Mannitol broth or Lactose broth by virtue of the presence of brilliant green and bile salts in the preparation.
EE Broth, Oxoid Composition
Typical Formula* | gm/litre |
Peptone | 10.0 |
Glucose | 5.0 |
Disodium hydrogen phosphate anhyd. | 6.45 |
Potassium dihydrogen phosphate | 2.0 |
Ox Bile purified | 20.0 |
Brilliant green | 0.0135 |
pH 7.2 ± 0.2 @ 25°C |
EE Broth, Oxoid Preparation:
Add 43.5g to 1 litre of distilled water. Distribute 100ml quantities in 250ml flasks and heat at 100°C for 30 minutes only. Cool rapidly in cold running water. This medium is heat sensitive. DO NOT AUTOCLAVE.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
Avoid overheating the medium, especially the double-strength broth.
Dimensions | 10 cm |
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Brand | Oxoid |
Estimated Delivery Time | 1-2 weeks |