Product Code | Description | Product Size | Quantity |
CM0485B | VIOLET RED BILE GLUCOSE AGAR 500g | – | 500g |
Violet Red Bile Glucose Agar 500g
Brand:
Thermo ScientificTM OxoidTM
Detect and enumerate Enterobacteriaceae in food products with Thermo Scientific™ Oxoid™ Violet Red Bile Glucose Agar (Dehydrated). This glucose-containing selective medium gives reproducible counts of typical colonies for food safety analysis. In the examination of food stuffs, detection of the Enterobacteriaceae that ferment glucose to produce acid and/or gas has been recommended.
Violet Red Bile Glucose Agar (Dehydrated), Oxoid Composition
Typical Formula* | gm/litre |
Yeast extract | 3.0 |
Peptone | 7.0 |
Sodium chloride | 5.0 |
Bile Salts No.3 | 1.5 |
Glucose | 10.0 |
Neutral red | 0.03 |
Crystal violet | 0.002 |
Agar | 12.0 |
pH 7.4 ± 0.2 @ 25°C |
Violet Red Bile Glucose Agar (Dehydrated), Oxoid Preparation:
Suspend 38.5g in 1 litre of distilled water. Bring to the boil. Continue to boil for 2 minutes or for the minimum time necessary to dissolve completely and ensure that there are no remaining flecks of unmelted agar. No further sterilisation is necessary or desirable. Mix well and dispense into tubes or dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C and use as freshly as possible.
Precautions
This medium is not completely specific for Enterobacteriaceae, other organisms may grow e.g. Aeromonas. The selective activity of this medium diminishes after 24 hours incubation and organisms previously suppressed may exhibit growth. Medium for the pour-plate procedure should be freshly prepared, cooled to 47°C and used within 3 hours.
Dimensions | 10 cm |
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Brand | Oxoid |
Estimated Delivery Time | 1-2 weeks |