Perfringens Agar Base TSC/SFP 500g

Selectively isolate and presumptively identify Clostridium perfringens in food samples with Thermo Scientific™ Oxoid™ Perfringens TSC Agar Base (Dehydrated). The medium can either be used on its own or with selective agents to make Tryptose Sulphite (TS) Agar, Tryptose Sulphite Cycloserine (TSC) agar or Shahadi Ferguson Perfringens (SPF) agar for the presumptive identification and enumeration of Clostridium perfringens.

Perfringens TSC/SFP Agar Base, Oxoid Composition

Agar Preparation:
To Prepare the Agar Base:
Suspend 23g in 500ml of distilled water and heat gently until the agar is completely dissolved. Sterilise by autoclaving at 121°C for 10 minutes. Allow the medium to cool to 50°C.

To Prepare Tryptose Sulphite Cycloserine Agar (TSC Agar):
To 500ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088) and 25ml of egg yolk emulsion (SR0047). Mix well and pour into sterile Petri dishes.

To Prepare Egg Yolk Free TSC Agar:
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of TSC supplement (SR0088). Mix well and pour into sterile Petri dishes.

To Prepare Shahidi-Ferguson Perfringens Agar (SFP Agar):
To 500 ml of Agar base cooled to 50°C add the rehydrated contents of 1 vial of SFP supplement (SR0093) and 25ml of egg yolk emulsion (SR0047) mix well and pour into sterile Petri dishes.

To Prepare Agar for an Overlay:
For TSC or SFP Agar used as an overlay, the egg yolk emulsion (SR0047) is omitted. Its inclusion does not improve the lecithinase reaction and diminishes the visibility of the colonies.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Black colonies appearing on these two media may be organisms other than Clostridium perfringens.