Brand:
Thermo ScientificTM OxoidTM
- Oxoid AFPA Medium Base is a selective identification medium for the detection of Aspergillus flavus and Aspergillus parasiticus.
- Also available: Membrane Clostridium Perfringens Selective Supplement, Part No. SR0188E.
AFPA Medium Base, Oxoid Composition
| Typical Formula * |
gm/litre
|
| Peptone |
10.0
|
| Yeast extract |
20.0
|
| Ferric ammonium Citrate |
0.5
|
| Dichloran |
0.002
|
| Agar |
15.0
|
| pH 6.3 ± 0.2 @ 25°C |
|
AFPA Medium Base, Oxoid Preparation:
Suspend 22.75g in 500ml (45.5g/l) of distilled water and heat to dissolve completely. Reconstitute one vial SR0078E per 500ml medium or one vial SR0078H per 2 litres medium, as directed. Add the vial contents to the AFPA Base. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Mix well and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.