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HomeMicrobiologyCulture Media Products Pre-Supplemented DG18 (ISO) Agar
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oxoid

Pre-Supplemented DG18 (ISO) Agar

RM1,242.00

Brand:

Thermo ScientificTM OxoidTM

Isolate and enumerate yeasts and molds associated with food spoilage with Thermo Scientific™ Oxoid™ Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base (Dehydrated) while adhering to BS ISO 21527-2: 2008. Due to the presence of antibiotic Chloramphenicol in the formulation, the medium provides selectivity against bacteria as well as reducing harmful chemical handling in the laboratory.

Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base (Dehydrated), Oxoid Composition

Typical Formula*

gm/litre

Casein Enzymatic digest

5.0

Glucose

10.0

Potassium dihydrogen phosphate

1.0

Magnesium sulphate

0.5

Dichloran (2,6-dichloro-4-nitroaniline)

0.002

Agar

15

Chloramphenicol 0.1
pH 5.6 ± 0.2 @ 25°C

 

Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base, Oxoid Preparation:
Suspend 15.8g per 500ml (31.6g/l) of distilled water and heat to dissolve completely. Before autoclaving add 220g of Glycerol per 1 litre of medium. Heat the complete solution gently to aid its dissolution of the glycerol. This has been found to be a key procedure to follow to allow proper dissolution of the glycerol. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes.

Where bacterial overgrowth occurs from certain foods (raw meats) chlortetracycline hydrochloride at 50mg/L (dissolved in water and filter sterilised) is recommended.

Storage instructions
Store the dehydrated medium at 10–30°C and use before the expiry date.

Precautions
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).

Pre-supplemented DG18 (ISO) Agar (CM1151) contains Chloramphenicol in the base powder and is therefore considered a toxic compound which should be handled in accordance with good industrial hygiene and safety practice. Always consult the MSDS before handling the powder.

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Size and packaging guidelines

Fermentum scelerisque hendrerit parturient nullam enim lobortis litora parturient dictumst.

Potenti a quisque tincidunt venenatis adipiscing parturient fermentum nisl tincidunt amentu.

Scelerisque conubia lobortis a condimentum ad eleifend dui integer maecenas habitant nostra.

Specification Chair Armchair Sofas
Height 37" 42" 42"
Width 26.5" 32.5" 142"
Depth 19.5" 22.5" 24.5"
Assembly Required No No Yes
Packaging Type Box Box Box
Package Weight 55 lbs. 64 lbs. 180 lbs.
Packaging Dimensions 27" x 26" x 39" 45" x 35" x 24" 46" x 142" x 25"
Size Guide
SKU: CM1151B Categories: Culture Media Products, Microbiology
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Description
Product Code Description Product Size Quantity
CM1151B Pre-Supplemented DG18 (ISO) Agar – 500g
Additional information
Dimensions 10 cm
Brand

Oxoid

Estimated Delivery Time

1-2 weeks

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Pre-Supplemented DG18 (ISO) Agar

Pre-Supplemented DG18 (ISO) Agar

RM1,242.00
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