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HomeMicrobiologyCulture Media Products Pre-Supplemented DRBC (ISO) Agar
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Pre-Supplemented DRBC (ISO) Agar

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Thermo ScientificTM OxoidTM

Isolate and enumerate yeasts and molds associated with food spoilage with Thermo Scientific™ Oxoid™ Pre-supplemented Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar Base (Dehydrated) while adhering to ISO 21527-1: 2008. Due to the presence of antibiotic Chloramphenicol in the formulation, the medium provides selectivity against bacteria as well as reducing harmful chemical handling in the laboratory.

Pre-supplemented Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar Base (Dehydrated), Oxoid Composition

Typical Formula*

gm/litre

Peptone

5.0

Glucose

10.0

Potassium dihydrogen phosphate

1.0

Magnesium sulphate

0.5

Dichloran

0.002

Rose-bengal

0.025

Agar

15.0

Chloramphenicol

0.1

pH 5.6 ± 0.2 @ 25°C

 

Pre-supplemented Dichloran Rose-Bengal Chloramphenicol (DRBC) (ISO) Agar, Oxoid Preparation:
Suspend 15.75g per 500ml (31.5g/l) of distilled water and heat to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes. It is advised to dispense the powdered medium into containers under an extraction hood due to the chloramphenicol in the medium base.

Storage instructions
Store the dehydrated medium at 10–30°C and use before the expiry date

Precautions
ROSE-BENGAL PHOTO-OXIDIZES TO FORM TOXIC COMPOUNDS. STORE PLATES OF THE MEDIUM IN THE DARK AND AVOID EXPOSURE TO LIGHT.
Some strains of fungi may be inhibited on this medium.
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).

Pre-supplemented DRBC (ISO) Agar (CM1149) contains Chloramphenicol in the base powder and is therefore considered a toxic compound which should be handled in accordance with good industrial hygiene and safety practice. Always consult the MSDS before handling the powder.

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SKU: CM1149B Categories: Culture Media Products, Microbiology
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  • Description
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Description
Product CodeDescriptionProduct SizeQuantity
CM1149BPre-Supplemented DRBC (ISO) Agar–500g
Additional information
Dimensions10 cm
Brand

Oxoid

Estimated Delivery Time

1-2 weeks

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