Brand:
Thermo ScientificTM OxoidTM
- Oxoid Anaerobe Basal Agar is used for growth of fastidious and other anaerobes.
- Also available N-S Anaerobe Selective Supplement, Part No. SR0107B, for use for nonsporforming anaerobes and G-N Anaerobe Selective Supplement, Part No. SR0108B, for use for gram-negative anaerobes.
Anaerobe Basal Agar, Oxoid Composition
| Typical Formula * | gm/litre |
| Peptone | 16.0 |
| Yeast extract | 7.0 |
| Sodium chloride | 5.0 |
| Starch | 1.0 |
| Dextrose | 1.0 |
| Sodium pyruvate | 1.0 |
| Arginine | 1.0 |
| Sodium succinate | 0.5 |
| L-cysteine HCl | 0.25 |
| Sodium bicarbonate | 0.4 |
| Ferric pyrophosphate | 0.5 |
| Haemin | 0.005 |
| Vitamin K | 0.0005 |
| Dithiothreitol | 0.25 |
| Agar | 12.0 |
| pH 6.8 ± 0.2 @ 25°C | |
Anaerobe Basal Agar, Oxoid Preparation:
Suspend 46g in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add 5-10% sterile Defibrinated Horse Blood SR0050.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium for upto 3 weeks at 2-8°C.