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HomeMicrobiologyCulture Media Products V.R.B.A. (IDF) 500g
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oxoid

V.R.B.A. (IDF) 500g

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Thermo ScientificTM OxoidTM

Isolate and enumerate coliforms in food, animal feed and environmental samples with Thermo Scientific™ Oxoid™ Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), while adhering to ISO 4832:2006 and ISO/TS 11133-2:2003. Due to the careful addition of selective inhibitory components, neutral red, crystal violet, and bile salts, the medium provides selectivity against non-target Gram-positive bacteria.

Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), Oxoid Composition

Typical Formula*

gm/ litre

Peptone

7.0

Yeast extract

3.0

Bile salts No. 3

1.5

Sodium chloride

5.0

Neutral red

0.03

Crystal violet

0.002

Lactose

10.0

Agar

12.0

pH 7.4 ± 0.2 @ 25°C

 

Violet Red Bile Lactose Agar (VRBL) (ISO) (Dehydrated), Oxoid Preparation:
Suspend 38.5g of VRBL Agar (ISO) dehydrated medium in 1 litre of distilled water. Bring to the boil while mixing. Continue to boil for up to 2 minutes or for the minimum time necessary to dissolve completely. DO NOT AUTOCLAVE. Cool to 44-47°C and use within 4 hours. Mix well before pouring.

Storage conditions and Shelf life
VRBL Agar (ISO) should be stored in the tightly capped original container at 10-30°C. When stored as directed, the un-opened product will remain stable until the expiry date printed on the packaging.

Locally prepared VRBL Agar (ISO) plates can be stored for up to 2 weeks when made from CM0968 according to the manufacturer’s instructions and stored at 2-8°C, out of direct sunlight. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

Precautions
VRBL Agar (ISO) should be prepared, cooled to 44-47°C and used within 4 hours.

VRBL Agar (ISO) is for laboratory use only. Do not use the dehydrated medium beyond the stated expiry date or if the product shows any sign of deterioration.

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SKU: CM0968B Categories: Culture Media Products, Microbiology
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  • Description
  • Additional information
Description
Product CodeDescriptionProduct SizeQuantity
CM0968BV.R.B.A. (IDF) 500g–500g
Additional information
Dimensions10 cm
Brand

Oxoid

Estimated Delivery Time

1-2 weeks

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V.R.B.A. (IDF) 500g

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