Brucella Medium Base 500g

For the preparation of serum-dextrose-antibiotic medium for the cultivation and isolation of Brucella using Brucella Selective Supplement SR0083 or SR0209. Without antibiotics, it may be used in conjunction with the Cruickshank dyestrip method for differentiation between strains.

Brucella Medium Base, Oxoid Composition

Brucella Medium, Oxoid Preparation:
Suspend 45 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add 5% of inactivated Horse Serum (i.e. Horse Serum held at 56°C for 30 minutes). Mix well before pouring.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.