Brucella Selective Supplement 1 x 10 Vials

Oxoid Brucella Selective Supplement is used for the isolation of Brucella spp.

• Add to Blood Agar Base No. 2, Part No. CM0271B; Columbia Blood Agar Base, Part No. CM0331B; or Brucella Medium Base, Part No. CM0169B
• Each vial supplements 500mL of medium

 Brucella Selective Supplement, Oxoid Composition

 Brucella Medium, Oxoid Preparation:
Reconstitute supplement as directed. Incubate for 10-15 minutes at 35°C. Mix thoroughly and immediately add the vial contents to 500 ml of sterile Oxoid Brucella Medium Base, cooled to 50°C together with 5-10% v/v sterile inactivated horse serum SR0035 and 1-5% w/v of a filter-sterilised 10% solution of dextrose. Mix well and pour into sterile petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.