Brand:
Thermo ScientificTM OxoidTM
Enumerate and differentiate bacteria in dairy products with this standard Thermo Scientific⢠Oxoid⢠China Blue Lactose Agar (Dehydrated). China Blue Lactose Agar, originally formulated by Brandl and Sobeck-skal, is a standard non-inhibitory solid medium used for the differentiation of lactose fermenters from non-lactose fermenters in milk.
The china blue in the medium serves as a pH indicator to differentiate lactose fermenters from non-lactose fermenters. The medium does not contain any inhibitory substances therefore all the organisms present in milk sample grow luxuriantly on this medium.
China Blue Lactose Agar Base, Oxoid Composition
| Typical Formula* |
gm/litre
|
| Peptone |
5.0
|
| `Lab-Lemcoâ powder |
3.0
|
| Lactose |
10.0
|
| Sodium chloride |
5.0
|
| China blue |
q.s.
|
| Agar |
12.0
|
| pH 7.0 ± 0.2 @ 25°C |
|
China Blue Lactose Agar, Oxoid Preparation:
Suspend 35g in 1 litre of distilled water. Boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
It is important to remember that Gram positive and negative cocci and bacilli can grow on this medium. Always confirm the organism morphology and Gram reaction.