Product Code | Description | Product Size | Quantity |
CM0419B | HEKTOEN ENTERIC AGAR 500g | – | 500g |
Hektoen Enteric Agar 500g
Brand:
Thermo ScientificTM OxoidTM
Isolate and differentiate Shigella and Salmonella spp. from enteric pathological specimens with Thermo Scientific™ Oxoid™ Hektoen Enteric Agar (Dehydrated). Hektoen Enteric Agar was developed by King and Metzger. When compared to other selective media this medium gives higher yield of Salmonella and Shigella, while inhibiting accompanying Gram-positive and other microorganisms.
Hektoen Enteric Agar, Oxoid Composition
Typical Formula* | gm/litre |
Proteose peptone | 12.0 |
Yeast extract | 3.0 |
Lactose | 12.0 |
Sucrose | 12.0 |
Salicin | 2.0 |
Bile salts No.3 | 9.0 |
Sodium chloride | 5.0 |
Sodium thiosulphate | 5.0 |
Ammonium ferric citrate | 1.5 |
Acid fuchsin | 0.1 |
Bromothymol blue | 0.065 |
Agar | 14.0 |
pH 7.5 + 0.2 @ 25°C |
Hektoen Enteric Agar, Oxoid Preparation:
Suspend 76g of the medium in 1 litre of distilled water and soak for 10 minutes. Heat gently and allow to boil for a few seconds to dissolve the agar. DO NOT AUTOCLAVE. Cool to 50°C and pour plates.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.
Precautions
Do not overheat the medium or hold it molten for long periods. Proteus species may resemble salmonellae or shigellae.
Further testing must be carried out to confirm the presumptive identification of organisms isolated on this medium.
Dimensions | 10 cm |
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Brand | Oxoid |
Estimated Delivery Time | 1-2 weeks |