Brand:
Thermo ScientificTM OxoidTM
- Oxoid Kanamycin Aesculin Azide Agar Base is a selective medium for the isolation of Enterococci in foodstuffs.
- Also available Kanamycin Sulphate Selective Supplement, Part No. SR0092E.
Kanamycin Aesculin Azide Agar Base, Oxoid Composition
| Typical Formula* |
gm/litre
|
| Tryptone |
18.8
|
| Yeast extract |
5.0
|
| Sodium chloride |
5.0
|
| Sodium citrate |
1.0
|
| Aesculin |
1.0
|
| Ferric ammonium citrate |
0.5
|
| Sodium azide |
0.15
|
| Starch |
0.6 |
| Mix for Streptococci |
0.6 |
| Â Agar |
10.0 |
| Final pH 7.0 ± 0.2 @ 25°C |
|
Kanamycin Aesculin Azide Agar, Oxoid Preparation:
Suspend 21.3g in 500ml of distilled water. Add one vial of Kanamycin Supplement (SR0092) reconstituted as directed. Bring to the boil. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared media at 2-8°C.
Precautions
Observe the hazard precautions regarding sodium azide when disposing of the medium.
There is no universal medium which will isolate all strains of enterococci.
Unless a presumptive count is acceptable all isolates should have their identity confirmed with further tests.