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HomeMicrobiologyCulture Media Products Kligler Iron Agar 500g, Oxoid
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oxoid

Kligler Iron Agar 500g, Oxoid

RM368.00

Brand:

Thermo ScientificTM OxoidTM

Oxoid Kligler Iron Agar is used for differential identificaton of Enterobacteriaceae.

Kligler Iron Agar, Oxoid Composition

Typical Formula*

gm/litre

`Lab-Lemco’ powder

3.0

Yeast extract

3.0

Peptone

20.0

Sodium chloride

5.0

Lactose

10.0

Glucose

1.0

Ferric citrate

0.3

Sodium thiosulphate

0.3

Phenol red

0.05

Agar

12.0

pH 7.4 ± 0.2 @ 25°C

 

Kligler Iron Agar, Oxoid Preparation:
Suspend 55g in 1 litre of distilled water. Bring to the boil to dissolve completely. Mix well and distribute into containers. Sterilise by autoclaving at 121°C for 15 minutes. Allow to set as slopes with 1 inch butts.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
It is essential that Kligler Iron Agar is examined and reported at 18-24 hours. Early or late readings will give false results.
KIA will grow both oxidative and fermentative organisms. Confusion will result if care is not taken to distinguish between the two groups.
Always use a straight wire to inoculate the butt, to avoid splitting the agar with a loop.
Pure cultures are essential to avoid misinterpretation.
Do not use screw-capped tubes or bottles for KIA medium. It is essential that air is freely available to growth on the slant.

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SKU: CM0033B Categories: Culture Media Products, Microbiology
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  • Description
  • Additional information
Description
Product CodeDescriptionProduct SizeQuantity
CM0033BKLIGLER IRON AGAR 500g–500g
Additional information
Dimensions10 cm
Brand

Oxoid

Estimated Delivery Time

1-2 weeks

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Kligler Iron Agar 500g, Oxoid

RM368.00
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