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HomeChemical LB broth (MILLER) for Microbiology (Merck)
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LB broth (MILLER) for Microbiology (Merck)

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Brand:

Merck

Description

LB-Broth is based on the formulation of MILLER (1972) supporting the growth of E. coli. Casein peptone and yeast extract supply essential growth factors, such as nitrogen, carbon, sulfur, minerals and vitamins.

Typical Composition of LB-Broth (Miller, Merck)

Typical Formula *
gm/litre
Yeast extract

5.0

Peptone from casein

10.0

Sodium chloride

10.0

Preparation of LB Broth (Miller)

Suspend 25 g LB-Broth in 1 litre of demineralized water and autoclave for 15 min at 121 °C.  at 25 °C (pH: 7.0 ± 0.2). The prepared broth is clear and yellowish-brown.

Experimental Procedure

According to appropriate use or purpose. Incubation: 24 h at 35-37 °C aerobically.

Product Specification and MSDS for LB Broth, Miller (Merck).

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SKU: 1102850500 Category: Chemical
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Description
Product CodeProduct NamePack SizeGradeStorage TemperatureShipment Temperature
1102850500LB broth (MILLER)500gMicrobiology+15°C to +25°CAmbient temperature
Additional information
Weight50 kg
Dimensions30 × 50 cm
Brand

Merck

Delivery Time

3 – 5 weeks

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LB broth (MILLER) for Microbiology (Merck)

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