|Product Code||Product Name||Pack Size||Grade||Storage Temperature||Shipment Temperature|
|1102850500||LB broth (MILLER)||500g||Microbiology||+15°C to +25°C||Ambient temperature|
LB broth (MILLER) for Microbiology (Merck)
LB-Broth is based on the formulation of MILLER (1972) supporting the growth of E. coli. Casein peptone and yeast extract supply essential growth factors, such as nitrogen, carbon, sulfur, minerals and vitamins.
Typical Composition of LB-Broth (Miller, Merck)
|Typical Formula *|
|Peptone from casein|
Preparation of LB Broth (Miller)
Suspend 25 g LB-Broth in 1 litre of demineralized water and autoclave for 15 min at 121 °C. at 25 °C (pH: 7.0 ± 0.2). The prepared broth is clear and yellowish-brown.
According to appropriate use or purpose. Incubation: 24 h at 35-37 °C aerobically.