Listeria Secondary Enrichment Supplement (UVMII) 1x10v

Oxoid Listeria Secondary Selective Enrichment Supplement, UVMII is used in a two-step selective enrichment (USDS-FSIS) method for the enrichment of Listeria spp.

• Add to Listeria Enrichment Broth Base (UVM formulation), Part No. CM0863B
• Each vial supplements 500mL of medium

Listeria Secondary Selective Enrichment Supplement (UVMII), Oxoid Composition

Listeria Secondary Selective Enrichment Medium, Oxoid Preparation:
Suspend 27.2g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C.
Aseptically add the contents of 1 vial of Listeria Secondary Selective Enrichment Supplement (UVM II) SR0143 reconsituted as directed. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140.
Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against listeriae.