Brand:
Thermo ScientificTM OxoidTM
Detect salmonellae including Salmonella arizonae with the diagnostic Thermo Scientific⢠Oxoid⢠Lysine Iron Agar (Dehydrated). This differential medium detects salmonellae (including lactose fermenting Salmonella arizonae) by lysine decarboxylase activity and H2S production.
Lysine Iron Agar, Oxoid Composition
| Typical Formula* |
gm/litre
|
| Bacteriological peptone |
5.0
|
| Yeast extract |
3.0
|
| Glucose |
1.0
|
| L-lysine |
10.0
|
| Ferric ammonium citrate |
0.5
|
| Sodium thiosulphate |
0.04
|
| Bromocresol purple |
0.02
|
| Agar |
14.5
|
| pH 6.7 ± 0.2 @ 25°C |
|
Lysine Iron Agar, Oxoid Preparation:
Suspend 34g in 1 litre of distilled water. Bring to the boil to dissolve completely. Dispense into tubes and sterilise by autoclaving at 121° C for 15 minutes. Cool the tubes in an inclined position to form slants with deep butts.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8° C.