Product Code | Description | Product Size | Quantity |
CM0191B | LYSINE MEDIUM 500g | – | 500g |
Lysine Medium 500g
Brand:
Thermo ScientificTM OxoidTM
Isolate and enumerate wild yeasts encountered in brewing while suppressing pitching yeasts with Thermo Scientific™ Oxoid™ Lysine Medium (Dehydrated). This complex, synthetic medium is based on the original formula of Morris & Eddy which utilizes the susceptibility of pitching yeasts to lysine to differentiate it from the wild yeast.
Lysine Medium, Oxoid Composition
Typical Formula* | gm/litre |
Glucose | 44.5 |
Potassium dihydrogen phosphate | 1.78 |
Magnesium sulphate | 0.89 |
Calcium chloride fused | 0.178 |
Sodium chloride | 0.089 |
Adenine | 0.00178 |
DL-methionine | 0.000891 |
L-histidine | 0.000891 |
DL-tryptophane | 0.000891 |
Boric acid | 0.0000089 |
Zinc sulphate | 0.0000356 |
Ammonium molybdate | 0.0000178 |
Manganese sulphate | 0.0000356 |
Ferrous sulphate | 0.0002225 |
Lysine | 1.0 |
Inositol | 0.02 |
Calcium pantothenate | 0.002 |
Aneurine | 0.0004 |
Pyridoxine | 0.0004 |
p-aminobenzoic acid | 0.0002 |
Nicotinic acid | 0.0004 |
Riboflavin | 0.0002 |
Biotin | 0.000002 |
Folic acid | 0.000001 |
Agar | 17.8 |
pH (see directions) |
Lysine Medium, Oxoid Preparation:
Suspend 6.6g in 100ml distilled water containing 1.0ml Potassium lactate SR0037. Bring to the boil to dissolve completely. Agitate frequently to prevent superheating. Cool to 50°C and add 0.1ml of lactic acid 10% SR0021 to adjust to pH 4.8 ± 0.2. Dispense into Petri dishes and remove surface moisture by drying at 37°C.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Dimensions | 10 cm |
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Brand | Oxoid |
Estimated Delivery Time | 1-2 weeks |