Brand:
Thermo ScientificTM OxoidTM
Oxoid MacConkey Agar No. 2 is a modification of MacConkey Agar No. 3 with addition of Bile Salts No. 2 for detection of Enterococci.
MacConkey Agar No. 2, Oxoid Composition
| Typical Formula* | gm/litre |
| Peptone | 20.0 |
| Lactose | 10.0 |
| Bile salts No.2 | 1.5 |
| Sodium chloride | 5.0 |
| Neutral red | 0.05 |
| Crystal violet | 0.001 |
| Agar | 15.0 |
| pH 7.2 ± 0.2 @ 25°C | |
MacConkey Agar, Oxoid Preparation:
Suspend 51.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.