Brand:
Thermo ScientificTM OxoidTM
Oxoid MacConkey Agar No. 2 is a modification of MacConkey Agar No. 3 with addition of Bile Salts No. 2 for detection of Enterococci.
MacConkey Agar No. 2, Oxoid Composition
| Typical Formula* |
gm/litre
|
| Peptone |
20.0
|
| Lactose |
10.0
|
| Bile salts No.2 |
1.5
|
| Sodium chloride |
5.0
|
| Neutral red |
0.05
|
| Crystal violet |
0.001
|
| Agar |
15.0
|
| pH 7.2 ± 0.2 @ 25°C |
|
MacConkey Agar, Oxoid Preparation:
Suspend 51.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.