Phosphate Buffered Saline Powder, pH 7.4, For Preparing 1 L solutions, Sigma

Application

• Phosphate buffered saline has been used in the washing solutions during immunofluorescence procedure.
• It has been used for the suspension of bacterial cells.
• It has been used during the fixation and immunostaining procedures.

Reconstitution

Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate-buffered saline (NaCl 0.138 M; KCl – 0.0027 M); pH 7.4, at 25 °C.