|Product Code||Description||Product Size||Quantity|
|CM0155B||SIMMONS CITRATE AGAR 500g||–||500g|
Simmons Citrate Agar 500g
Thermo ScientificTM OxoidTM
Simmons Citrate Agar (Dehydrated), Oxoid Composition
|Ammonium dihydrogen phosphate|
|Sodium ammonium phosphate|
|Sodium citrate, tribasic|
|pH 7.0 ± 0.2 @ 25°C|
Simmons Citrate Agar (Dehydrated), Oxoid Preparation:
Suspend 23g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
It is important not to carry over any nutrients into the citrate medium because this will result in false positive tests. Dilute the inoculum in saline before inoculating the citrate medium to avoid a carry-over of other carbon sources.