| Product Code | Description | Product Size | Quantity |
| CM0155B | SIMMONS CITRATE AGAR 500g | – | 500g |
Simmons Citrate Agar 500g
Brand:
Thermo ScientificTM OxoidTM
Differentiate members of the family Enterobacteriaceae based on the utilization of citrate with Thermo Scientific™ Oxoid™ Simmons Citrate Agar (Dehydrated). The medium is a solidified form of Koser citrate medium which suffered from the disadvantage that false appearance of growth occurred when large inocula were employed. Simmons modified Koser’s formulation by adding agar and bromothymol blue. It complies with the recommendations if the APHA.
Simmons Citrate Agar (Dehydrated), Oxoid Composition
| Typical Formula* | gm/litre |
| Magnesium sulphate | 0.2 |
| Ammonium dihydrogen phosphate | 0.2 |
| Sodium ammonium phosphate | 0.8 |
| Sodium citrate, tribasic | 2.0 |
| Sodium chloride | 5.0 |
| Bromothymol blue | 0.08 |
| Agar | 15.0 |
| pH 7.0 ± 0.2 @ 25°C |
Simmons Citrate Agar (Dehydrated), Oxoid Preparation:
Suspend 23g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
It is important not to carry over any nutrients into the citrate medium because this will result in false positive tests. Dilute the inoculum in saline before inoculating the citrate medium to avoid a carry-over of other carbon sources.
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Additional information
| Dimensions | 10 cm |
|---|---|
| Brand | Oxoid |
| Estimated Delivery Time | 1-2 weeks |
