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HomeMicrobiologyCulture Media Products T.C.B.S Cholera Medium 500g
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oxoid

T.C.B.S Cholera Medium 500g

RM283.00

Brand:

Thermo ScientificTM OxoidTM

Selectively isolate pathogenic Vibrio species with Thermo Scientific™ Oxoid™ TCBS (Thiosulfate Citrate Bile Salts Sucrose) Cholera Medium (Dehydrated). The medium has shown to be the most satisfactory and reproducible formula regardless of the complex composition. Unlike Lauryl Sulphate Tellurite Agar it does not require further additives or aseptic additions of blood. It also possesses superior growth characteristics for Vibrio species, compared with tellurite media. While inhibiting non-vibrios, it promotes rapid growth of pathogenic vibrios after overnight incubation at 35°C.

TCBS (Thiosulfate Citrate Bile Salts Sucrose) Cholera Medium (Dehydrated), Oxoid Composition

Typical Formula*

gm/litre

Yeast extract

5.0

Bacteriological peptone

10.0

Sodium thiosulphate

10.0

Sodium citrate

10.0

Ox Bile

8.0

Sucrose

20.0

Sodium chloride

10.0

Ferric citrate

1.0

Bromothymol blue

0.04

Thymol blue

0.04

Agar

14.0

pH 8.6 ± 0.2 @ 25°C

 

TCBS (Thiosulfate Citrate Bile Salts Sucrose) Cholera Medium (Dehydrated), Oxoid Preparation:
Suspend 88g in 1 litre of distilled water. Boil to dissolve the medium completely. DO NOT AUTOCLAVE.
Pour plates without further heating and dry before use.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
The identification of the various Vibrio species on TCBS Medium is presumptive and further tests are required for confirmation.
Yellow colonies on TCBS Medium will give unsatisfactory oxidase reactions.
Colonies taken from TCBS Medium are `sticky’ and react poorly in slide agglutination tests. Subculture to nutrient agar is required before slide agglutination tests can be carried out.
Some strains of Vibrio vulnificus produce better recovery at 30°C.

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SKU: CM0333B Categories: Culture Media Products, Microbiology
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  • Description
  • Additional information
Description
Product CodeDescriptionProduct SizeQuantity
CM0333BT.C.B.S CHOLERA MEDIUM 500g–500g
Additional information
Dimensions10 cm
Brand

Oxoid

Estimated Delivery Time

1-2 weeks

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T.C.B.S Cholera Medium 500g

RM283.00
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