Urea Agar Base 500g, Oxoid

Oxoid Urea Agar Base (Christensen Agar Base) is used for detection of urease-producing microorganisms.

Urea Agar Base, Oxoid Composition

Urea Agar Preparation:
Suspend 2.4g in 95ml of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 115°C for 20 minutes. Cool to 50°C and aseptically introduce 5ml of sterile 40% Urea Solution SR0020. Mix well, distribute 10ml amounts into sterile containers and allow to set in the slope position.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
The alkaline reaction produced in this medium after prolonged incubation may not be caused by urease activity. Check using medium without urea.
Do not heat or reheat the medium because urea decomposes very easily.
For the detection of urease-positive Proteae the reaction must be read within the first 2-5 hours of incubation.