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Selectively enrich Salmonella spp. from food and environmental specimens with Thermo Scientific™ Oxoid™ Rappaport-Vassiliadis Enrichment Broth (Dehydrated). Rappaport-Vassiliadis Enrichment Broth selects for Salmonella species by exploiting their characteristics when compared with other Enterobacteriaceae, such as the ability to survive at relatively high osmotic pressure, multiply at relatively low pH values, be relatively more resistant to malachite green, and have relatively less demanding nutritional requirements.

Rappaport-Vassiliadis Enrichment Broth (Dehydrated), Oxoid Composition

THIS MEDIUM IS VERY HYGROSCOPIC AND MUST BE PROTECTED FROM MOISTURE.
The quantities given for the formula as classically described made 1110ml of medium. They have been published this way in the Oxoid literature to coincide with the scientific literature.

The directions for reconstituting Oxoid Rappaport-Vassiliadis (RV) Enrichment Broth follow usual Oxoid practice and specify the weight needed for 1 litre of medium.

Rappaport-Vassiliadis Enrichment Broth (Dehydrated), Oxoid Preparation:
Add 30g (the equivalent weight of dehydrated medium per litre) to 1 litre of distilled water. Heat gently until dissolved completely. Dispense 10ml volumes into screw-capped bottles or tubes and sterilise by autoclaving at 115°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
RV Broth should not be used if Salmonella typhi is suspected.
Note the difference in weight between the classical formula on the label and the reduced weight per litre, using anhydrous magnesium chloride.

Cultivate streptococci, Neisseria and other fastidious organisms with Thermo Scientific™ Oxoid™ Brain Infusion Broth (Dehydrated). The medium is highly nutritious and well buffered to support the growth of wide variety of organisms. It is recommended for blood culture work for the isolation and cultivation of pathogenic fungi.

Brain Infusion Broth, Oxoid Composition

Brain Infusion Broth, Oxoid Preparation:
Dissolve 37g in 1 litre of distilled water. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store tubed or bottled medium in the dark and below 20°C.

Oxoid Campylobacter Selective Supplement, Blaser-Wang is used to prepare of Blaser-Wang Selective Medium for the isolation of Campylobacter while inhibiting growth of Candida albicans.

• Add to Columbia Blood Agar Base, Part No. CM0271B
• Each vial supplements 500mL of medium

Campylobacter Selective Supplement, Blaser-Wang, Oxoid Composition

Campylobacter Selective Medium, Oxoid Preparation:
Reconstitue one vial as directed add the contents of one vial to 500ml of sterile nutrient medium cooled to approximately 50°C prepared from Columbia Agar or Blood Agar Base No.2, with 10% defibrinated horse/sheep blood or 5-7% laked horse blood SR0050, SR0051 or SR0048. Mix gently and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Thermo Scientific™ Oxoid Listeria Primary Enrichment Supplement (UVMI) is a primary selective enrichment supplement for the isolation of listeria when used with Listeria Enrichment Broth Base (UVM Formulation) CM0863.

Oxoid Listeria Primary Enrichment Supplement (UVMI), Oxoid Composition

Listeria Primary Selective Enrichment Medium, Oxoid Preparation:
Suspend 27.2g in 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C.
Aseptically add the contents of 1 vial of Listeria Primary Selective Enrichment Supplement (UVM I) SR0142 reconsituted as directed. Mix well and distribute into sterile containers.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Note the precautions stated under Listeria Selective Medium (Oxford) CM0856 and SR0140.
Broth cultures are more dangerous than colonies on agar plates.
Store prepared medium away from light. Acriflavine can photo-oxidise to form inhibitory compounds against listeriae.

This product has been discontinued, please refer to SR0258E

Oxoid Brilliance Listeria Differential Supplement is added to Brilliance Listeria Agar Base, Mfr. No. CM1080E and Mfr. No. CM1080B, a chromogenic medium for the isolation and differentiation of Listeria

• Add to Brilliance Listeria Agar Base, Part No. CM1080B
• Each vial supplements 500mL of medium

Oxoid Brilliance Listeria Differential Supplement, Oxoid Composition

Brilliance Listeria Agar, Oxoid Preparation:
Suspend 33.6g of Brilliance Listeria Agar Base (CM1080) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Brilliance Listeria Selective Supplement, reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

The Oxoid Peptone Bacteriological is for use whenever a high quality bacteriological peptone is required.

Enrich and isolate halophilic organisms, especially staphylococci, using Thermo Scientific™ Oxoid™ Salt Meat Broth Tablets. Salt Meat Broth Tablets are specially designed for the isolation of staphylococci from grossly contaminated specimens such as feces, particularly during the investigation of staphylococcal food poisoning. The medium is highly sensitive as it can detect even low numbers of staphylococci from samples having large proportions of heterogeneous microbial flora.^1,2

• Quantity: 100 tablets/bottle

Salt Meat Broth Tablets, Oxoid Composition

Salt Meat Broth, Oxoid Preparation:
Add 2 tablets to 10ml of distilled water in an appropriately sized test tube and soak for 5 minutes. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature.

The Thermo Scientific™ Oxoid Tryptone ia a pancreatic digest of casein for use where high performance and uniformity of composition are of paramount importance.