Brand:
Thermo ScientificTM OxoidTM
Isolate and presumptively identify enterococci / Group D streptococci with Thermo Scientific⢠Oxoid⢠Bile Aesculin Agar (Dehydrated). Bile Aesculin Agar is designed to differentiate between Group D streptococci and non-Group D streptococci.
Group D streptococci hydrolyse aesculin to form aesculetin and dextrose. Aesculin hydrolysis is indicative of a positive result. Bile salts in the medium inhibit Gram-positive bacteria other than enterococci/ Group D streptococci.
Bile Aesculin Agar, Oxoid Composition
| Typical Formula* |
gm/litre
|
| Peptone |
14.0
|
| Bile salts |
15.0
|
| Ferric citrate |
0.5
|
| Aesculin |
1.0
|
| Agar |
15.0
|
| pH 7.1 ± 0.2 @ 25°C |
|
Bile Aesculin Agar, Oxoid Preparation:
Suspend 44.5g in 1 litre of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.