Brand:
Thermo ScientificTM OxoidTM
This product has been discontinued. Please contact us for more information.
Oxoid GBS Agar Base (Group B Streptococci Medium) (Islam) is used for the isolation and detection of Group B Streptococci in clinical specimens.
GBS Agar Base (Islam), Oxoid Composition
| Typical Formula* |
gm/litre
|
| Proteose peptone |
23.0
|
| Soluble starch |
5.0
|
| Sodium dihydrogen phosphate |
1.5
|
| Di-sodium hydrogen phosphate |
5.75
|
| Agar |
10.0
|
| pH 7.5 ± 0.1 @ 25°C |
|
GBS Agar Base (Group B Streptococci Medium) (Islam), Oxoid Preparation:
Suspend 45.2g in 1 litre of distilled water and bring to the boil to disolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 50ml of sterile inactivated Horse Serum. Mix well and pour into Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.
Precautions
The medium must be at its correct pH value to ensure good pigmentation.
Some strains of Group B streptococci do not produce pigmented colonies.
Do not hold the molten medium any longer than necessary.