|GIOLITTI-CANTONI BROTH 500g
Giolitti-Cantoni Broth 500g
Thermo ScientificTM OxoidTM
An anaerobic enrichment broth for Staphylococcus aureus.
Giolitti-Cantoni Broth, Oxoid Composition
|pH 6.9 ± 0.2 @ 25°C
Giolitti-Cantoni Broth, Oxoid Preparation:
Suspend 54.2g in one litre of distilled water and heat gently to dissolve. Dispense 19ml amounts into 20 x 200mm test tubes and sterilise by autoclaving at 121°C for 15 minutes. Cool rapidly then aseptically add to each tube 0.3ml of a sterile solution of Potassium tellurite 3.5% SR0030.
The medium requires the addition of a 3.5% solution of Potassium tellurite when there is a direct addition of 1g of the sample to 19mls of broth. This level of Potassium tellurite is necessary to suppress the high numbers of contaminating organisms that could be expected.
The use of a diluted solution of Potassium tellurite is applicable when a 1 in 10 dilution of the food sample is carried out. In such cases the SR0030 should first be diluted 1 in 10 with sterile distilled water.
The addition of 0.1% Tween 80 can be recommended in order to improve recovery of heat injured Staphylococcus aureus cells e.g. from milk powder. 1g of Tween 80 should be added to 1 litre of CM0523 prior to autoclaving.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.