Brand:
Thermo ScientificTM OxoidTM
Detect thermophilic anaerobic organisms causing sulphide spoilage in food with Thermo Scientific⢠Oxoid⢠Iron Sulphite Agar (Dehydrated). The medium is a modification of Cameron Sulphite Agar developed by the National Canners Association of America1. Iron Sulphite Agar has a reduced concentration of sodium sulphite to allow improved detection of some strains of Clostridium sporogenes.
Iron Sulphite Agar, Oxoid Composition
| Typical Formula* |
gm/litre
|
| Tryptone |
10.0
|
| Sodium sulphite |
0.5
|
| Iron (III) citrate |
0.5
|
| Agar |
12.0
|
| pH 7.1 ± 0.2 @ 25°C |
|
Iron Sulphite Agar, Oxoid Preparation:
Suspend 23.0g in 1 litre of distilled water and boil to dissolve completely. Sterilise by autoclaving for 15 minutes at 121°C. Mix well before pouring.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium below 25°C.
Precautions
The blackening reaction is only presumptive evidence of clostridial growth. Confirmation tests must be carried out to identify the organism growing in the medium.