Brand:
Thermo ScientificTM OxoidTM
- Oxoid Perfringens OPSP Agar Base is used for the enumeration of Clostridium perfringens in foods.
- lso available Perfringens OPSP Supplement A, Part No. SR0076E and Perfringens OPSP Supplement B, Part No. SR0077E.
Perfringens OPSP Agar Base, Oxoid Composition
| Typical Formula* |
gm/litre
|
| Tryptone |
15.0
|
| Yeast extract |
5.0
|
| Soya peptone |
5.0
|
| Liver extract |
7.0
|
| Ferric ammonium citrate |
1.0
|
| Sodium metabisulphite |
1.0
|
| Tris buffer |
1.5
|
| Agar |
10.0
|
| pH 7.3 ± 0.2 @ 25°C |
|
Perfringens OPSP Agar, Oxoid Preparation:
Suspend 22.8g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C and aseptically add the contents of one vial each of Perfringens Agar (OPSP) supplements A and B (SR0076 and SR0077) which have been rehydrated as directed. Mix well and pour into sterile dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
The production of black colonies on this medium is a presumptive identification of Clostridium perfringens. Further identification tests must be carried out.