Product Code | Description | Product Size | Quantity |
CM0543B | PERFRINGENS AGAR 500g | – | 500g |
Pefringens Agar 500g
Brand:
Thermo ScientificTM OxoidTM
- Oxoid Perfringens OPSP Agar Base is used for the enumeration of Clostridium perfringens in foods.
- lso available Perfringens OPSP Supplement A, Part No. SR0076E and Perfringens OPSP Supplement B, Part No. SR0077E.
Perfringens OPSP Agar Base, Oxoid Composition
Typical Formula* | gm/litre |
Tryptone | 15.0 |
Yeast extract | 5.0 |
Soya peptone | 5.0 |
Liver extract | 7.0 |
Ferric ammonium citrate | 1.0 |
Sodium metabisulphite | 1.0 |
Tris buffer | 1.5 |
Agar | 10.0 |
pH 7.3 ± 0.2 @ 25°C |
Perfringens OPSP Agar, Oxoid Preparation:
Suspend 22.8g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C and aseptically add the contents of one vial each of Perfringens Agar (OPSP) supplements A and B (SR0076 and SR0077) which have been rehydrated as directed. Mix well and pour into sterile dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Precautions
The production of black colonies on this medium is a presumptive identification of Clostridium perfringens. Further identification tests must be carried out.
Dimensions | 10 cm |
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Brand | Oxoid |
Estimated Delivery Time | 1-2 weeks |