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HomeMicrobiologyCulture Media Products Pefringens Agar 500g
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Pefringens Agar 500g

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Brand:

Thermo ScientificTM OxoidTM

  • Oxoid Perfringens OPSP Agar Base is used for the enumeration of Clostridium perfringens in foods.
  • lso available Perfringens OPSP Supplement A, Part No. SR0076E and Perfringens OPSP Supplement B, Part No. SR0077E.

Perfringens OPSP Agar Base, Oxoid Composition

Typical Formula*

gm/litre

Tryptone

15.0

Yeast extract

5.0

Soya peptone

5.0

Liver extract

7.0

Ferric ammonium citrate

1.0

Sodium metabisulphite

1.0

Tris buffer

1.5

Agar

10.0

pH 7.3 ± 0.2 @ 25°C

 

Perfringens OPSP Agar, Oxoid Preparation:
Suspend 22.8g in 500ml of distilled water and bring gently to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C and aseptically add the contents of one vial each of Perfringens Agar (OPSP) supplements A and B (SR0076 and SR0077) which have been rehydrated as directed. Mix well and pour into sterile dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
The production of black colonies on this medium is a presumptive identification of Clostridium perfringens. Further identification tests must be carried out.

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SKU: CM0543B Categories: Culture Media Products, Microbiology
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  • Description
  • Additional information
Description
Product CodeDescriptionProduct SizeQuantity
CM0543BPERFRINGENS AGAR 500g–500g
Additional information
Dimensions10 cm
Brand

Oxoid

Estimated Delivery Time

1-2 weeks

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Pefringens Agar 500g

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