Pre-Supplemented DG18 (ISO) Agar

Isolate and enumerate yeasts and molds associated with food spoilage with Thermo Scientific™ Oxoid™ Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base (Dehydrated) while adhering to BS ISO 21527-2: 2008. Due to the presence of antibiotic Chloramphenicol in the formulation, the medium provides selectivity against bacteria as well as reducing harmful chemical handling in the laboratory.

Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base (Dehydrated), Oxoid Composition

Pre-supplemented Dichloran-Glycerol 18% (DG18) (ISO) Agar Base, Oxoid Preparation:
Suspend 15.8g per 500ml (31.6g/l) of distilled water and heat to dissolve completely. Before autoclaving add 220g of Glycerol per 1 litre of medium. Heat the complete solution gently to aid its dissolution of the glycerol. This has been found to be a key procedure to follow to allow proper dissolution of the glycerol. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C, mix well and pour into sterile Petri dishes.

Where bacterial overgrowth occurs from certain foods (raw meats) chlortetracycline hydrochloride at 50mg/L (dissolved in water and filter sterilised) is recommended.

Storage instructions
Store the dehydrated medium at 10–30°C and use before the expiry date.

Precautions
The dichloran compound used in this medium is Botran® 2,6-Dichloro-4-Nitro-Analine (CAS: 99-30-9).

Pre-supplemented DG18 (ISO) Agar (CM1151) contains Chloramphenicol in the base powder and is therefore considered a toxic compound which should be handled in accordance with good industrial hygiene and safety practice. Always consult the MSDS before handling the powder.